Сборник от електронната юбилейна научна конференция с международно участтие “Животновъдната наука-предизвикателства и иновации, 5 ноевмври, 2020 г., Костинброд
Печат Авангард Прима, София, ISBN 978-619- 90918-5-2, eISBN 978-619- 90918-5-2, ИЖН - Костинброд
Печат Авангард Прима, София, ISBN 978-619- 90918-5-2, eISBN 978-619- 90918-5-2, ИЖН - Костинброд
Proceedings of the online anniversary scientific conference with international participation “Animal Science- Challenges and Innovations”, 5 November, 2020, Kostinbrod
Printed by Avangard Prima, Sofia, ISBN 978-619- 90918-5-2, eISBN 978-619- 90918-5-2, IAS- Kostinbrod
Printed by Avangard Prima, Sofia, ISBN 978-619- 90918-5-2, eISBN 978-619- 90918-5-2, IAS- Kostinbrod
PCR-RFLP ANALYSIS AND GENETIC DIVERSITY OF GDF9 IN TWO INDIGENOUS BULGARIAN SHEEP BREEDS
Автори / Authors
Milena Bozhilova-Sakova, Ivona Dimitrova
Abstract
GDF9 (growth differentiating factor 9) is a genetic marker related to fecundity in sheep. The aim of the study was to investigate the genetic diversity of the GDF9 gene in two indigenous Bulgarian sheep breeds by PCR-RFLP analysis. DNA samples were collected and genotyped, and genotype and allele frequencies were estimated. The results revealed breed-specific distributions of GDF9 genotypes and alleles; in the Cooper-Red Shumen sheep breed two genotypes were identified, while in the Karakachan sheep breed all possible genotypes were identified, indicating higher variability in the latter.
Ключови думи / Keywords
Cooper-Red Shumen, Karakachan, GDF9, PCR-RFLP, genetic diversity
Bozhilova-Sakova, M., & Dimitrova, I. (2020). PCR-RFLP Analysis and Genetic Diversity of GDF9 in Two Indigenous Bulgarian Sheep Breeds. Proceedings of the online anniversary scientific conference „Animal Science – Challenges and Innovations”, 5 November, 2020, Kostinbrod, Institute of Animal Science – Kostinbrod, Sofia, Bulgaria, ISBN 978-619- 90918-5-2, eISBN 978-619- 90918-5-2, pp. 257–261.
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